sin membrane window  (Norcada Inc)

Journal: Nature Communications

Article Title: Self-assembling peptides imaged by correlated liquid cell transmission electron microscopy and MALDI-imaging mass spectrometry

doi: 10.1038/s41467-019-12660-1

Figure Lengend Snippet: MALDI-IMS of LCTEM chips after assembly as a liquid cell containing KLDL or cycKLDL peptide. a–f Analysis of LCTEM chip with 10-nm-thick silicon nitride (SiN x ) membrane window, coated with KLDL peptide. a Optical image of a square window of SiN x LCTEM chip. b Map of laser spots with raster size of 50 µm. c Intensity map of merged KLDL and cycKLDL signal. d Intensity map of KLDL signal only. ROI for magnification in white box. e Intensity map of cycKLDL signal only. f Magnified ROI showing 2D map pixels. g–l Analysis of chip coated with cycKLDL peptide. g Optical image of square window of SiN x LCTEM chip. h Map of laser spots with raster size of 50 µm. i Intensity map of merged KLDL and cycKLDL signal. j Intensity map of KLDL signal only. k Intensity map of cycKLDL signal only. ROI for magnification in white box. l Magnified ROI showing 2D map pixels. m Color intensity is displayed as 0–100% of total intensity on a logarithmic scale for each filter within the analyzed region. n Total averaged MALDI mass spectra from each chip plotted as absolute intensity vs. m/z . Filter (with manually defined range represented as ±) for KLDL [M + Na + ] + at 1493 ± 4.5 m/z is shown in red. Filter for cycKLDL [M + H + ] + at 2140 ± 4.5 m/z is shown in blue. Intensity is calculated as integrated intensity for mass range. Source data are provided as a Source Data file

Article Snippet: All following LCTEM/MALDI-IMS experiments were performed with chips having 50-nm-thick, 200 µm × 50 µm SiN x membrane windows (Hummingbird Scientific, Lacey, WA, USA).

Techniques: